Fig. 2: Pharmacological inhibition of XPO1 induces cell death, p53 upregulation/phosphorylation, and caspase-dependent apoptosis, in HTLV-1-transformed cell lines. | Blood Cancer Journal

Fig. 2: Pharmacological inhibition of XPO1 induces cell death, p53 upregulation/phosphorylation, and caspase-dependent apoptosis, in HTLV-1-transformed cell lines.

From: XPO1 inhibitors represent a novel therapeutic option in Adult T-cell Leukemia, triggering p53-mediated caspase-dependent apoptosis

Fig. 2

A Simple western analysis for p53 and phosphorylated p53 was performed on whole-cell lysates after overnight incubation of MT-2 or MT-4 cells with different concentrations of KPT-330. The graphs show mean of p53 and phosphorylated p53 expression relative to vinculin expression with SEM (n = 4). Data are normalized to 1 µM KPT-330 within each separate experiment. RM one-way ANOVA, with Geisser-Greenhouse correction. *p = 0.0332; **p = 0.0021, ***p = 0.0002, and ****p < 0.0001. B Cell viability relative to untreated control as determined by MTT of HTLV-1 transformed cell lines in the presence of different concentrations KPT-330. Error bars represent standard error of mean (n = 3). C MT-2 or MT-4 cells were treated with DMSO or different concentrations of KPT-330 and after 3 days analyzed by Annexin V/PI flow cytometry. Error bars represent standard error of mean (SEM) (n = 3).

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