Fig. 6: BCR signaling activated RNA pol II and increased Mcl-1 levels; this was blocked by TG02. | Blood Cancer Journal

Fig. 6: BCR signaling activated RNA pol II and increased Mcl-1 levels; this was blocked by TG02.

From: The multi-kinase inhibitor TG02 induces apoptosis and blocks B-cell receptor signaling in chronic lymphocytic leukemia through dual mechanisms of action

Fig. 6

A CLL cells were incubated with TG02 or Lcki for 1 h before stimulating with anti-IgM for 1 h and 24 h. The phosphorylation RNA pol II and the levels of anti-apoptotic proteins were evaluated by immunoblotting. The mean CLL cell viability of three individual experiments assessed at 24 h is shown below the image. B Inhibiting Lck had minimal toxicity on the CLL cells. CLL cells were incubated with Lcki for 1 h before stimulating with anti-IgM for 24 h. The percentage of surviving cells with or without Lcki was compared in groups without (white bars) and with (gray bars) anti-IgM stimulation (mean ± SEM, n = 8).

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