Fig. 2: Downregulation of LCK and LYN kinases inhibits NVP-BEP800 efficiency on T and B lymphoblast cell lines. | Blood Cancer Journal

Fig. 2: Downregulation of LCK and LYN kinases inhibits NVP-BEP800 efficiency on T and B lymphoblast cell lines.

From: HSP90 inhibitor NVP-BEP800 affects stability of SRC kinases and growth of T-cell and B-cell acute lymphoblastic leukemias

Fig. 2

Western blot shows low levels of expression of p-LCK and total LCK after the viral transduction of Jurkat cells with shLCK (a), or p-LYN and total LYN after the viral transduction of Raji cells with shLYN (b). Cell growth assay shows no sensitivity of shLCK Jurkat cells (c) and shLYN Raji cells (d) to NVP-BEP800. 2 × 105 viable cells (trypan blue negative) are seeded. Cells are treated with NVP-BEP800 (1 µM) at days 0 and 5. Data are shown as mean; n = 3 biological replicates for T-ALL and n = 4 biological replicates for B-ALL. P value measured by one-way Anova test with Tukey’s multiple comparison test; ****P < 0.0001; ns, non-significant.

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