Fig. 2: γH2AX phosphorylation status before and after X-ray irradiation.
From: Potential role of STAG1 mutations in genetic predisposition to childhood hematological malignancies
Cells were seeded at different, previously established, concentrations, in order to perform the experiments in an exponential growth phase (0.1 × 106/ml for CTR3-8F, 0.22 × 106/ml for CTR6-9M and 0.18 × 106/ml for M-STAG1). A shows that γH2AX phosphorylation status of M-STAG1 is higher at basal conditions and increases during timepoints compared to control LCLs (T0: 2.8X, p < 0.001; T24: 2.7X, p < 0.0001; T48: 3.5X, p < 0.0001; MFI M-STAG1 over MFI control LCLs). γH2AX phosphorylation remains at higher levels also after irradiation [3Gy] (B, T24: 2.6X, ns; T48: 6.3X, p < 0.0001 [3Gy]; MFI M-STAG1 over MFI control LCLs, normalized on the percentage of γH2AX+ cells). The percentage of pH2AX++ subpopulation, recognized only for M-STAG1 either at basal level (10.5X, p < 0.01; percentage of pH2AX++ M-STAG1 cells over percentage of pH2AX++ control LCLs cells) or after irradiation (T0: 6.2X, p < 0.01; T24: 10.6X, p < 0.0001; T48: 14.1X, p < 0.0001; percentage of pH2AX++ cells M-STAG1 over percentage of pH2AX++ cells controls’ LCLs), shows the same trend (C). (n = 3 replicates. Statistical analysis performed by one-way Anova with Bonferroni’s multiple comparison correction. * <0.05; **<0.01 ***<0.001; ****<0.0001).
