Fig. 2: HMMR splice variant transcripts are frequently and recurrently expressed in different subpopulation of cells from MM patients. | Blood Cancer Journal

Fig. 2: HMMR splice variant transcripts are frequently and recurrently expressed in different subpopulation of cells from MM patients.

From: Identification of disease-related aberrantly spliced transcripts in myeloma and strategies to target these alterations by RNA-based therapeutics

Fig. 2

This figure displays HMMR splice variant expressions at a single-cell level. MM or HD cells were sorted by index sorting followed by RT-PCR DNA fragment analyses and capillary electrophoresis. A, B display HMMR splice variant expression at the single-cell level in MM-PCs and myeloid cells, while C and D display the expression of those variants in autologous MM BMSCs and HD BMSCs. A, C show HMMR splice variant co-expression levels. In these figures, the x axes display cells and the y axes relative fluorescent units (RFU) of the PCR products. PCR product RFU = lg(RFU). RFU is a unit of measurement calculated relative to the size standards included in each reaction. B, D The overall distribution of the HMMR splice variants in different subpopulations of MM and HD samples are presented as heatmaps. The color scale for expression values is shown on the right.

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