Table 1 Univariate and multivariate logistic regression analysis of those immunophenotypic features of BM leukemia cells associated with NPM1 mutation (A) and FLT3-ITD (B) from AML patients (n = 377).

From: Baseline immunophenotypic profile of bone marrow leukemia cells in acute myeloid leukemia with nucleophosmin-1 gene mutation: a EuroFlow study

 

AML-NPM1mut vs. AML-NPM1wt and APL

(A) Variables and leukemia cell subsets

Univariate analysis

 

Multivariate analysis

 

OR

95% CI

p-value

OR

95% CI

p-value

CD34+ and/or CD117+HLADR+ leukemia cells

<26.5% of all leukemia cells

2.0

1.4–2.5

<0.001

   

CD34 (<35%)

4.8

2.6–8.6

<0.001

7.7

3.5–17.0

<0.001

CD33 (>96%)

1.4

1.0–2.0

0.04

   

CD105 (<9.5%)

0.4

0.2–0.6

0.001

   

HLA-DR (<97%)

0.3

0.3–0.7

0.001

0.2

0.1–0.5

<0.001

CD15 (>6.6%)

0.3

0.1–0.6

<0.001

0.2

0.1–0.5

<0.001

CD7 (>3%)

1.5

1.0–2.2

0.02

2.0

1.1–3.8

0.02

CD56 negative

0.3

0.1–0.6

0.002

   

NuTdT negative

0.2

0.1–0.5

<0.001

0.2

0.1–0.5

0.002

Neutrophil-committed leukemia cells

>21.5% of all leukemia cells

1.6

1.2–2.2

0.008

-

-

-

CD34 (<5%)

4.0

2.4–6.6

<0.001

7.0

2.9–17.5

<0.001

CD71 (<70%)

2.5

1.6–3.9

<0.001

3.0

1.1–7.9

0.02

CD105 (>3%)

5.1

2.7–9.8

<0.001

4.8

1.9–12.4

0.001

CD64 (<30%)

4.3

2.5–7.5

<0.001

4.4

1.8–10.9

0.001

CD13 (<92%)

3.2

2.0–5.1

<0.001

-

-

-

CD56 (>5%)

5.6

2.1–14.5

<0.001

-

-

-

Monocytic-committed leukemia cells

Any asynchronous pattern

6.5

3.8–11.1

<0.001

-

-

-

Asynchronous CD300e+CD14- profile

85.0

12.0–610

<0.001

49.0

3.7–641

0.004

Asynchronous CD35+CD14- profile

11.4

5.5–23

<0.001

-

-

-

CD34+ (<3.8%)

3.7

2.4–5.7

<0.001

-

-

-

Any asynchronous pattern plus CD34+ (<3.8%)

34.3

11.0–108

<0.001

223.9

3.7–641.5

<0.001

CD117 (<5.9%)

3.7

2.1–6.5

0.001

-

-

-

CD13 (<77%)

4.3

2.7–6.8

<0.001

0.2

0.04–1.0

0.05

CD123 (>83%)

2.9

1.8–4.5

<0.001

0.3

0.1–0.8

0.02

CD15+ (>77%)

3.4

2.2–5.4

<0.001

-

-

-

CD36 (>87%)

3.2

2.0–5.1

<0.001

-

-

-

FLT3-ITD+ vs. FLT3-ITD-

(B) Variables and leukemia cell subsets

      

AML-NPM1mut patients

CD34+ and/or CD117+HLADR+ leukemia cells

CD34+ (>3%)

5.3

1.9–14.8

0.001

-

-

-

CD38 (<95%)

5.6

2.2–14.1

<0.001

0.1

0.01–0.8

0.03

CD7 (>55%)

5.4

2.2–13.9

<0.001

7.2

1.0–48.5

0.04

CD25 (>25%)

7.1

1.3–37.5

0.02

-

-

-

Neutrophil-committed leukemia cells

CD117 (<69%)

5.7

2.1–15.5

0.001

9.4

2.7–32.4

<0.001

CD123 (>84%)

4.6

1.7–12.6

0.003

7.6

2.2–26.0

0.001

CD13 (>56%)

2.6

1.0–6.8

0.05

-

-

-

AML-NPM1wt patients

 % total BM blasts (>40%)

3.7

1.2–11.6

0.02

-

-

-

CD34+ and/or CD117+HLADR+ leukemia cells

CD34+ (<57%)

4.3

1.6–11.5

0.004

3.8

1.0–15.3

0.05

CD25 (>10%)

6.9

1.4–33.8

0.01

7.9

1.5–40.3

0.01

  1. OR odds ratio, CI confidence interval.
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