Fig. 3: Targeted nanoparticles induce the highest percentage of apoptotic cell death. | Blood Cancer Journal

Fig. 3: Targeted nanoparticles induce the highest percentage of apoptotic cell death.

From: BCMA-targeted bortezomib nanotherapy improves therapeutic efficacy, overcomes resistance, and modulates the immune microenvironment in multiple myeloma

Fig. 3

Apoptotic cell death induction in MM.1S cells after treatment with BTZ, BTZ-NPs and BCMA-BTZ-NPs for 24 h, measured by Annexin V/PI assay: A representative FACs images and B bar plot. The percentage of mitochondrial membrane depolarization (MMP) was measured by JC1 staining: C representative FACs images and D bar plot. Evaluation of Caspase Activities in MM.1S cells: Bar diagram showing progressive increased activity in BTZ, BTZ-NPs and BCMA-BTZ-NPs, respectively. for E Caspase 3, F Caspase 8, and G Caspase 9 after 24 h of treatment. Mean ± SD of triplicate cultures, ***p < 0.001, **p < 0.01, significance was analyzed by Student’s t-test.

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