Fig. 6: Real-time in vivo fluorescence imaging in multiple myeloma subcutaneous tumor-bearing mice at different time points.

A Schematic representation of DiR dye- loaded non-targeted and targeted nanoparticles IV injection into multiple myeloma subcutaneous tumor-bearing mice model. B In vivo accumulation of DiR dye-loaded non-targeted (upper panel) and BCMA-targeted (lower panel) nanoparticles monitored at 12 h, 24 h, and 48 h. Bio distribution of DiR-dye loaded C non-targeted and D targeted nanoparticles to liver, lung, kidney, and tumor isolated 12 h, 24 h, and 48 h after IV injection, with quantitative assessment for E non-targeted and F targeted group. The respective fluorescence intensity was measured with the unit Radiance (p/s/cm2 /sr) for Region of Interest (ROI) quantified using AMI viewer image software. G In vivo tumor tissue localization of DiR-loaded non-targeted (upper panel) and BCMA-targeted (lower panel) nanoparticles was assessed by fluorescence imaging of tumor tissue histology from tumors extracted 48 h after IV injection. Each animal image shown here represents a single replicate from the corresponding group. Mean ± SD of 2-5 independent experiments performed in triplicate. Each organ image represents a single replicate extracted from the corresponding mouse group.