Fig. 3: FL-PDLS immune profile. | Blood Cancer Journal

Fig. 3: FL-PDLS immune profile.

From: Patient-derived follicular lymphoma spheroids recapitulate lymph node signaling and immune profile uncovering galectin-9 as a novel immunotherapeutic target

Fig. 3

A Differential gene expression analysis from microarray data obtained from public repositories (detailed in supplemental methods) showed up-regulation of several immune regulators in FL-LN (n = 427) compared to a normal tonsil (n = 30). Fold changes (FC) are indicated and red color means statistical significance (unpaired nonparametric t test, Mann–Whitney). B Heatmap representing the percentage of positive cells assessed by flow cytometry for the immune regulators on CD4 and CD8 T cells of day 3-and day 7 FL-PDLS (n = 3–11). Results were compared with PBMCs from healthy donors (n = 4). C Uniform Manifold Approximation and Projection (UMAP) plot for day 3-FL-PDLS autologous CD3+ cells based on the expression of activation and exhaustion markers assessed by flow cytometry and colored by cluster identity (left panel). Percentage distribution of those clusters (middle panel). UMAP plots show the distribution of PD-1, TIM-3, LAG-3, and ICOS expression (right panel). D Average expression levels of each protein represented on CD3+ clusters (n = 6). E Day 3-FL-PDLS autologous CD3+ phenotypes based on flow cytometry CCR7 and CD45RA expression. F Percentage of TFH (CXCR5+FoxP3−), TREG (CXCR5−FoxP3+), and TFR (CXCR5+FOXP3+) out of CD4+ population on day 3-FL-PDLS. Patients are identified by the origin of the FL sample.

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