Fig. 2: TC-RK induced disease mirrors features of human multiple myeloma.

a Representative serum electrophoresis of immunoglobulins from TC-RK and control mice. b Representative quantification of immunoglobulin isotypes in plasma samples of five diseased TC-RK mice by flow cytometry-based multiplex immunoassay. c Red blood cell count (RBC) of diseased TC-RK mice and indicated controls (n = 3–6 per genotype). d Representative images of H&E staining from bone marrow of five-month-old RK and TC-RK mice (scale bars: overview = 600 μm, detailed images: 60 μm) e Representative images of H&E staining from kidneys of diseased TC-RK mice and indicated diseased or aged control mice (scale bars: 100 μm). f Kaplan-Meier OS analysis of Rag2^ko mice (n = 13) transplanted with TC-RK splenocytes derived from four different sick donor mice compared to Rag2^ko control mice. g Spleen weight of Rag2^ko mice (n = 8) transplanted with TC-RK splenocytes at end point and aged-matched Rag2^ko mice (n = 4). h Percentages of GFP^hiCD19^neg cells and GFP⁺CD19⁺ cells of viable cells from BM, SP, PB and LI isolated from diseased Rag2^ko mice (n = 9–10 per organ) transplanted with TC-RK splenocytes derived from three different donor mice. Statistical analysis was performed using Student’s t-test and one-way ANOVA with Tukey correction for multiple comparison. P values are indicated in respective graphs and all data are presented as mean ± standard deviation.