Fig. 3: Inference of lineage distribution of mCAs by comparison of mCA cell fraction against B-cell fraction.

Data are shown for canonical CLL-associated mCAs (A), CLL-driver mCAs (B), and lymphoid mCAs (C), the classification of which is detailed in the “Methods” section. Each point represents a single individual with HC-MBL from the Mayo Clinic MBL Biobank. For all individuals shown, flow cytometry was performed on frozen peripheral blood mononuclear cells (PBMCs) and DNA was extracted from PBMCs sampled on the same day as for flow cytometry. Data points with mCA cell fraction of 0 indicate individuals in whom the specified mCA type was not detected. Data points above the dashed red line indicate individuals in whom the fraction of cells containing a canonical CLL-associated mCA exceeds the B-cell fraction, suggesting the presence of the mCA beyond the B-cell lineage and origin prior to B-cell lineage commitment.