Fig. 2: Soluble factors from osteoclasts promote leukemia cell proliferation and survival.
From: Targeting osteoclasts for treatment of high-risk B-cell acute lymphoblastic leukemia
A Proteomic analysis of the supernatant from differentiated RAW264.7 cells compared to controls (n = 3 per group). The value refers to the label-free quantitation intensity. B Multidimensional scaling analysis of global gene expression in leukemia cells cultured with conditioned media (CM) (n = 4) and control media (n = 4). C Volcano plot highlighting the top differentially expressed genes of leukemia cells following culture with CM and control media. D, E Gene set enrichment analysis comparing RNA sequencing-generated global transcriptomes of leukemia cells in CM (n = 4) vs. leukemia cells in control media (n = 4) by D KEGG pathway and E GO Biological Process annotations. Significance was assigned by q-value < 0.05. F Number of leukemia cells after culture with CM and control media for 3 days (n = 4 per group). G Cell cycle analysis of leukemia cells cultured with CM and control media for 3 days (n = 4 per group). H Apoptosis assay of leukemia cells in CM and control media for 3 days (n = 4 per group). F–H Error bars are represented as mean ± SEM. *p < 0.05, ***p < 0.001, ****p < 0.0001 or the precise p-value where indicated.
