Fig. 1: FasL AM MPBCs treatment significantly reduces CD3+ cells without compromising the quantity and quality of CD34+ cells. | Bone Marrow Transplantation

Fig. 1: FasL AM MPBCs treatment significantly reduces CD3+ cells without compromising the quantity and quality of CD34+ cells.

From: Short treatment of peripheral blood cells product with Fas ligand using closed automated cell processing system significantly reduces immune cell reactivity of the graft in vitro and in vivo

Fig. 1

a FasL AM MPBCs treatment process performed using the Fresenius Kabi LOVO functionally closed automated cell processing system, designed for HSCT. MPBCs graft characterization following (b–f) 100 ng/ml FasL AM treatment or (g–k) 400 ng/ml FasL AM treatment. Percentage of (b, g) CD3+ and (d, i) CD34+ cells per total CD45+ population. Percentage of annexin V positive (c, h) CD3+ and (e, j) CD34+ cells per 7AAD− population. f, k Percentage of multi potent stem and progenitor cells (CD34+CD38neg/low) per total CD34+7AAD− population. b–e: N = 16, f: N = 9, g–k: N = 3. Mean ± SEM, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001; Paired T-test.

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