Fig. 4: FasL AM treatment of MPBCs increases their phagocytosis and reduces proinflammatory cytokine secretion by activated macrophages. | Bone Marrow Transplantation

Fig. 4: FasL AM treatment of MPBCs increases their phagocytosis and reduces proinflammatory cytokine secretion by activated macrophages.

From: Short treatment of peripheral blood cells product with Fas ligand using closed automated cell processing system significantly reduces immune cell reactivity of the graft in vitro and in vivo

Fig. 4

100 ng/ml FasL AM treated and non-treated MPBCs were incubated with activated macrophages at different ratios. Percentage of high phagocytic (CFSE+high) macrophages per total CD11b+ population as well as secretion of TNFα, IL-6 and IL-8 were evaluated. Percentage of high phagocytic (CFSE+high) macrophages: (a) Representative flow cytometry dot plots, (b) results of the representative experiment are presented as mean ± SEM of technical duplicates, *P ≤ 0.05; ***P ≤ 0.001, Multiple unpaired T-test. c The effect of engulfment of apoptotic cells by activated macrophages on TNFα, IL-8 and IL-6 secretion. Results of a representative experiment are presented as Mean ± SEM of technical duplicates. Macrophages: cell ratio 1:5; *P ≤ 0.05; Multiple unpaired T-test.

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