Table 3 Advantages and limitations of VCN quantification techniques.

From: Harmonisation of quality control tests for academic production of CAR-T cells: a position paper from the WP-bioproduction of the UNITC consortium

Test

Advantages

Limitations

qPCR

Well-established and validated method. Relatively fast and accessible.

qPCR is an indirect method that requires the use of standards for quantification. It can be subject to variations depending on amplification efficiency and biases introduced during analysis.

ddPCR

Absolute quantification of VCN, eliminating the need for a standard curve. High precision, sensitivity, and reproducibility. Less prone to variations than qPCR, allowing detection of copy number variations even in heterogeneous samples.

Expensive method requiring specialized equipment. May be slower than qPCR in some laboratories.

  1. qPCR PCR Quantitative, ddPCR Droplet Digital PCR.
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