Fig. 4
Super enhancer inhibitors suppress osteosarcoma migration and invasion. a U2OS cells were cultured in regular mediumto 100% confluence and then further cultured another 24 h in low-serum (2%) medium. Then scratches were induced and cells were treated with 50 nmol·L-1 THZ1 and 250 nmol·L-1 JQ1 in low-serum medium.Cell images were then acquired to monitor the cell migration after 24 h. b Quantification of cell migration (a). c U2OS osteosarcoma cells were treated with 500 nmol·L-1 JQ1, 100 nmol·L-1 THZ1 or DMSO for 24 h, then 50 000 cells were seeded into the Matrigel Invasion Chamber. The cell images and invasive cells were acquired and quantified after 16 h. d Quantification of invasive cells (c). n = 3, **P < 0.01, ***P < 0.001.
