Fig. 2
PU.1 is essential for both OC lineage commitment and differentiation. a Dorsoventral whole body radiographs of 4-week-old male littermate Pu.1fl/fl;CtskCre+ (Pu.1ΔOC/ΔOC) mice and Pu.1fl/fl controls. Image is representative of n = 3 pairs. Scale bar = 1 cm. b Lateral (top) and dorsoventral (bottom) radiographs of the skulls of 4-week-old littermate Pu.1ΔOC/ΔOC mice and Pu.1fl/fl controls. Image is representative of n = 3 pairs. Scale bars = 1 cm. c Lateral femoral and tibial radiographs and bone mineral density (BMD) quantification of the distal metaphyseal and diaphyseal regions of the femurs of 4-week-old littermate Pu.1ΔOC/ΔOC mice and Pu.1fl/fl controls (n = 3). Scale bars = 2.5 mm. d Same as c except from 8-day-old littermate Pu.1ΔOC/ΔOC mice and Pu.1fl/fl controls (n = 4 Pu.1fl/fl, n = 3 Pu.1ΔOC/ΔOC). Scale bars = 1 mm. e Histologic images and histomorphometry of the distal femoral metaphyses of the mice in d (n = 3). Scale bars = 50 μm. f Flow plots and quantification of CD11b-/loLy6Chi osteoclast precursors (OCPs) in the BM of 6–8-week-old Pu.1ΔMP/ΔMP mice and Pu.1fl/fl controls (n = 4). g Images and quantification of TRAP staining of in vitro differentiated OCPs from f (n = 4). For all figures and subfigures, data are represented as the mean ± S.D. for all bar and line graphs and all images are representative. In addition, for all figures and subfigures, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
