Fig. 3
PU.1 is associated with cis-acting elements critical for OC differentiation and bone remodeling irrespective of RANKL signaling. a Graphical representation of the distribution of PU.1 OC ChIP-Seq peaks throughout the mouse genome. b ChIP-Seq tag density coverage of murine BMDM H3K27Ac active enhancer marks (red) and murine BMDM H3K4me3 active promoter marks (green) ± 3 kilobases (kb) from PU.1 OC peak centers. c Treeview plot of genome-wide PU.1 myeloid precursor (MP) tags ± 3 kb from PU.1 OC peak centers. d Venn diagram depicting the number of genes in MPs and OCs with PU.1 peaks. e GSEA plot of an OC differentiation gene set significantly enriched in genes with PU.1 OC peaks using our murine MP and OC microarray data (n = 3). Heatmap (right) indicating MP and OC expression of genes in the gene list. f RT-qPCR analysis of genes on the GSEA gene list essential for OC function. Gene expression is shown for wild-type (WT) MPs and OCs and Pu.1 KO OCs (n = 3). g Treeview plot of PU.1 MP and OC tags ± 500 base pairs from the transcriptional start sites (TSS) of all genes on the GSEA list. h Depiction of MP and OC PU.1 ChIP-Seq peaks ± 3 kb from the TSS of Acp5, Ctsk, and Oscar. Conventional ChIP validation of PU.1 binding to the starred sites is shown (bar graphs, n = 3).
