Fig. 5
From: Histone demethylase LSD1 regulates bone mass by controlling WNT7B and BMP2 signaling in osteoblasts
Wnt7b and Bmp2 are directly regulated by LSD1 in osteoblasts. a, b Genomic tracks display ChIP-seq data using H3K4me2 across the promoter region of Wnt7b (a) and Bmp2 (b). c, d ChIP-qPCR analysis of H3K4me2 enrichment in the Wnt7b (c) and Bmp2 (d) promoter regions in calvarial cells. n = 3 for each group, all data represent means ± SD ANOVA followed by Tukey’s post hoc test was performed, *P < 0.05, ***P < 0.001. e, f ChIP-qPCR analysis of LSD1 enrichment in the Wnt7b (e) and Bmp2 (f) promoter regions in calvarial cells. n = 3 for each group, all data represent means ± SD Unpaired t-test was performed, *P < 0.05, **P < 0.01, ***P < 0.001. g Immunohistochemistry for WNT7B and BMP2 showing increased expression in a coronal section of the proximal tibias of 1-week-old Lsd1 prx1 mice compared to littermate controls. The images are representative of three mice per group. Scale bar, upper panel: 200 μm, lower panel: 40 μm.
