Fig. 6

WNT7B related mTOR signaling and BMP2 related BMP signaling contribute to the increased bone mass in Lsd1^prx1 mice. a Lsd1 ^fl/fl calvarial cells derived from P3 mice were infected with Egfp or Cre virus followed by culture in osteoblast differentiation medium for 7 days. WNT7B and pS6K1 levels were measured by western blot. Results are representative of two independent experiments. b–f Femurs from 6 weeks female mice (n = 4–6) were analyzed by μCT. b 3D reconstructions of the trabecular bone and midshaft cortical bone. Quantitative parameters of trabecular bone (c–e) and cortical bone (f) were analyzed. ANOVA followed by Tukey’s post hoc test was performed. **P < 0.01. Scale bar, 0.5 mm. g Lsd1 ^fl/fl calvarial cells derived from P3 mice were infected with Egfp or Cre virus followed by culture in osteoblast differentiation medium for 7 days. LSD1, p-SMAD1/5/8, and BMP2 levels were measured by western blot. Results are representative of two independent experiments. h–l Femurs from 9 weeks Lsd1^prx1 and Lsd1^fl/fl male mice (n = 4–6) after BMP inhibitor LDN-193185 injection for 4 weeks were analyzed by μCT. h 3D reconstructions of the trabecular bone and midshaft cortical bone. Quantitative parameters of trabecular bone (I–k) and cortical bone (l) were analyzed. ANOVA followed by Tukey’s post hoc test was performed. *P < 0.05. Scale bar in h: 0.5 mm.