Fig. 1
BMP signalling in Gli1+ lineage-derived osteoprogenitors in adult sutures. a Whole-mount LacZ staining (blue) of calvarial bones from Gli1-LacZ mice at postnatal 2 weeks (P2w), 1 month (P1m), and 6 months (P6m). The arrows indicate the posterior frontal suture. b H-E staining and tdTomato (red) visualisation of the posterior frontal suture and sagittal suture of Gli1-CreERT2;tdTomato mice 1 day post induction with tamoxifen (1dpt). Arrows indicate Gli1+ cells. c Bmpr1a immunostaining (green) and tdTomato visualisation (red) of the sagittal suture mesenchyme of Gli1-CreERT2;tdTomato mice 2 days (2 dpt), 14 days (14 dpt), and 28 days (28 dpt) post induction. Red arrows indicate Gli1+ cells; green arrows indicate Bmpr1a+ cells; yellow arrows indicate co-localization. The boxed area is shown at higher magnification to the right. Broken lines indicate the outline of the suture. Scale bars in a, 1 mm; b and c, 100 µm
