Fig. 5
From: Erythropoietin modulates bone marrow stromal cell differentiation
Osteoclasts in Tg6- and ΔEpoRE mice. a Representative images of TRAP stained osteoclasts in the femur sections of 11-week-old wt and Tg6-mice. Arrows indicate purple stained osteoclasts along the surface of trabecular bones. b Images of in vitro osteoclast differentiation of bone marrow cells of wild-type (wt) and Tg6-mice using M-CSF and RANKL. Osteoclasts appear after 4–5 days and were stained for TRAP. c TRAP-stained sections of femurs of 11-week-old wild-type (wt) and ΔEpoRE-mice showing osteoclasts. Arrows indicate purple stained osteoclasts along the surface of trabecular bone. d In vitro osteoclast differentiation from the bone marrow of wt and ΔEpoRE-mice identified by TRAP staining. e–h Number of osteoclasts on the bone surface of wt and Tg6-mice (e), number of osteoclasts in the bone marrow cultures of wt and Tg6-mice (f), number of osteoclasts on the bone surface of wt and ΔEpoRE-mice (g), and number of osteoclasts in the bone marrow cultures of wt and ΔEpoRE-mice (h) analyzed using ImageJ. i TRAP stained femur sections of 8-week-old wt and ΔEpoRE-mice receiving 1200 U·kg–1 of EPO or saline for 10 days. Arrows indicate purple stained osteoclasts along the trabecular bone surface. j Enumeration of osteoclasts in the wt and ΔEpoRE-mice receiving EPO or saline. k Relative expression of Cathepsin K levels in the whole bone marrow determined by real-time PCR. (n = 5/group, *P < 0.05, **P < 0.01, ***P < 0.001)
