Fig. 6
From: Erythropoietin modulates bone marrow stromal cell differentiation
Elevated EPO and lack of EPOR signaling inhibit BMP2-induced ectopic bone formation. a Micro-CT images of ectopic bone formed by BMP2 in collagen scaffolds transplanted in wild-type (wt) and Tg6-mice 4 weeks post transplantation. Shown are ossicles formed in wt mice (top) and in Tg6-mice (bottom), and inner trabecular bone (right) and cortical bone (left). b Micro-CT images of ectopic bone formed by BMP2 in collagen scaffolds transplanted in wild-type (wt) and ΔEpoRE-mice 4 weeks post transplantation. Shown are ossicles formed in wt mice (top) and in ΔEpoRE-mice (bottom), and inner trabecular bone (right) and cortical bone (left). c H&E staining of decalcified ossicles formed in the wt and Tg6-mice, showing bone as pink colored regions, adipocytes as white circular regions and marrow as blue regions. d H&E stained images of ossicles formed in wt and ΔEpoRE-mice bone, marrow and adipocytes. e, f Micro-CT quantification of trabecular (e) and cortical (f) volume of the ectopic bone formed in wt and Tg6-mice. g Quantification of marrow adipocytes in the wt and Tg6-ossicle sections. h Space occupied by marrow (excluding adipocytes and bones). i Number of TRAP stained osteoclasts in the wt and Tg6-ossicles. j, k Micro-CT quantification of trabecular (j) and cortical (k) volume of the BMP2-induced ectopic bone formed in wt and ΔEpoRE-mice. l Quantification of marrow adipocytes in the wt and ΔEpoRE-ossicle sections. m Space occupied by marrow (excluding adipocytes and bone). n Number of TRAP stained osteoclasts in the wt and ΔEpoRE-ossicles. Quantification of adipocytes, marrow space and osteoclast numbers were performed using ImageJ analysis. (n = 4–5/group, *P < 0.05, **P < 0.01)
