Fig. 7
From: Erythropoietin modulates bone marrow stromal cell differentiation
In vivo bone formation potential of BMSCs from Tg6- and ΔEpoRE-mice transplanted in immunocompromised mice. a Micro-CT images of ectopic bone formed by the differentiation of BMSCs from wild-type (wt) and Tg6-mice 4 weeks post transplantation. Shown are wt BMSC ossicles (top) and Tg6-BMSC ossicles (bottom), and inner trabecular bone (right) and cortical bone (left). b Micro-CT images of ectopic bone formed by BMSCs from wild-type (wt) and ΔEpoRE-mice. Shown are wt BMSC ossicles (top) and ΔEpoRE-BMSC ossicles (bottom), and inner trabecular bone (right) and cortical bone (left). c, d H&E staining of wt, Tg6 (c), and ΔEpoRE (d) BMSC ossicles showing cortical bone (CB), trabecular bone (TB), adipocytes (A), gelfoam (G), and bone marrow (BM). e, f Quantification of trabecular (e) and cortical bone volume (f) of wt and Tg6-BMSC ossicles. g, h Area of adipocytes (g) and bone marrow (h) in wt and Tg6–BMSC ossicles quantified using ImageJ software analysis. i Quantification of the number of osteoclasts present in the ossicles developed from the wt and Tg6-BMSCs. j, k Micro-CT quantification of trabecular (j) and cortical bone volume (k) of ossicles developed from the differentiation of wt and ΔEpoRE-BMSCs. l, m Area of adipocytes (l) and bone marrow (m) inside the wt and ΔEpoRE-ossicles. n Quantification of osteoclasts present on the wt and ΔEpoRE-BMSC ossicles. (n = 4/group, **P < 0.01, ***P < 0.001)
