Fig. 2
BMSCsadipo and BMSCsosteo progenitors exhibit differential responses to insulin. Representative western blot to evaluate insulin-stimulated (100 nmol·L–1, 15 min) phosphorylation of AKT (p-S473AKT) and total AKT, INSRβ a in undifferentiated BMSCsadipo and BMSCsosteo and b BMSCsadipo and BMSCsosteo differentiated cells in adipogenic conditions (n = 3). Insulin-stimulated glucose uptake using a 14C glucose tracer after a 24-h incubation c in undifferentiated (baseline) BMSCs and d BMSCs differentiated under adipogenic conditions from BMSCsadipo and BMSCsosteo. Data are presented as the mean of glucose uptake normalized to the baseline of murine 3T3-L1 fibroblasts as a positive control ± SEM from three independent experiments. (*P < 0.05, **P < 0.01: BMSCsadipo vs BMSCsosteo, two-tailed unpaired Student’s t test; #P < 0.05: nonstimulated vs insulin-stimulated cells). Profile of neutral lipids in BMSCsadipo, BMSCsosteo, and 3T3-L1 cells under insulin stimulation (100 nmol·L–1) using thin layer chromatography (TLC): e densitometry of triglycerides (TG) and f the the fatty acid (FA) content in BMSC differentiated cells under adipogenic conditions. Densitometry of 14C FA (g) and 14C TG (h) incorporation in BMSC differentiated cells under adipogenic conditions. Data are presented as the mean of F.C. ± SEM from three independent experiments. (n = 3) (*P < 0.05, **P < 0.01: BMSCsadipo vs BMSCsosteo, one-way ANOVA)
