Fig. 5 | Bone Research

Fig. 5

From: Evading the host response: Staphylococcus “hiding” in cortical bone canalicular system causes increased bacterial burden

Fig. 5

Live–dead confocal microscopy. Confocal fluorescent microscopy of cortical allograft, stainless steel disc, or cancellous allograft at POD 56 following inoculation with sterile saline, or 1 × 102 CFU of S. aureus Xen36, magnification ×100. The commercially available Live/Dead BacLight Bacterial Viability Kit (Thermo Fischer Scientific, Canoga Park, CA) was utilized. SYTO9 dye stains nucleic acids in intact cell membranes, representing live bacteria. Propidium iodide die stains nucleic acids in ruptured cell membranes, representing dead bacteria. The comparison of the live stain in the cortical allograft reveals qualitatively more green stain/100 μm in the infected compared to the sterile group, whereas the live stains in the stainless steel and cancellous allograft appear more similar between infected and sterile groups. While the intent of the SYT09 dye is to stain microbial cells, host cells may represent a confounding factor and cautions the interpretation of this data

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