Fig. 2
From: MEF2C regulates osteoclastogenesis and pathologic bone resorption via c-FOS
MEF2CΔMX mice show increased bone mass with decreased osteoclast numbers. a μCT analysis of femurs from 16-week-old male MEF2CΔMX KO (n = 7) and littermate control WT mice (n = 9). Right panels show the indicated parameters in distal femurs. Bone volume/tissue volume ratio (BV/TV), trabecular thickness (Tb.Th), trabecular numbers (Tb.N), and porosity were determined by μCT analysis. b Histomorphometry analysis of the distal femur of 16-week-old male mice. Representative images showing TRAP-positive, multinucleated osteoclasts (red). Scale bars, 500 μm. Right panels show number of osteoclasts per bone surface (N.Oc/BS), osteoclast surface area per bone surface (Oc.S/BS) and eroded surface per bone surface (ES/BS). c Dynamic bone histomorphometry analysis of the distal femur. Scale bars, 50 μm. Representative images showing casein incorporation into newly calcifying bone. Right panels showed mineral apposition rate (MAR) and bone formation rate (BFR/BS). Data are shown as mean ± SD. Statistics used: a, b, c Welch’s t-test. NS; not significant, *P < 0.05, **P < 0.01
