Fig. 4

MEF2C regulates expression of FOS and c-FOS-target genes in RANKL-stimulated human OCPs. a Heatmap showing relative expression (z-score) of 202 genes differentially expressed with P < 0.01 in RANKL-stimulated MEF2C KD cells versus RANKL-stimulated control cells from three biological replicates. b Enriched transcription factor binding motifs in region ±2 kb relative to transcription start site in RANKL-regulated MEF2C-dependent genes, by gene set enrichment analysis (GSEA). c Volcano plot of RNA-seq analysis of differentially expressed genes in human macrophages transduced with control or MEF2C#2 siRNAs. MEF2C and eight other genes obtained from b are marked. Of note, LEF1 and FOXF2 were filtered out due to low expression level (CPM < 3). Red dots, genes with q value < 0.05 (34 genes). d Cumulative values for FOS in RANKL-stimulated samples from RNA-sequencing with three biological replicates. Data are shown as mean ± SD