Fig. 3

Interfacial stability of adsorbed FN on different substrates in a cell culture environment. a Immunofluorescence staining of adsorbed FN (cyan) on different SF substrates with and without BMSCs cultured for 24 h, together with F-actin (red) and nuclei (blue). The purple arrows indicate the detached FN underlying the cells. b Ratio of the FN dark area underlying the cell outline to the cell area. c Ratio of FN intensity underlying the cell outline to that of the background. Error bars represent one standard deviation. (*P < 0.05 and **P < 0.01)