Fig. 5 | Bone Research

Fig. 5

From: The molecular conformation of silk fibroin regulates osteogenic cell behavior by modulating the stability of the adsorbed protein-material interface

Fig. 5

YAP/TAZ and RUNX2 nuclear translocation in BMSCs on different SF substrates. a Representative mosaic immunofluorescence images of YAP/TAZ (red), RUNX2 (yellow), and nuclei (blue) in BMSCs at 24 h and day 3. b, c The nuclear-cytoplasmic ratios of (b) YAP/TAZ and (c) RUNX2 in BMSCs analyzed from the immunofluorescence images. d Relative fluorescence intensity per cell of RUNX2 at day 3. e Single-cell scatter plots of the YAP/TAZ nuclei/cytoplasm ratio as a function of the RUNX2 nuclei/cytoplasm ratio at day 3. f–m The mRNA expression levels of (f–g) Yap, (h–i) Ankrd1, (j–k) Ctgf, and (l–m) Runx2 in BMSCs at 24 h and day 3. For each box plot, the box boundaries represent the 25th–75th percentiles, and the whiskers represent the min and max values. The central line and cross represent the median value and mean, respectively. Error bars represent one standard deviation. (*P < 0.05 and **P < 0.01)

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