Fig. 6 | Bone Research

Fig. 6

From: The molecular conformation of silk fibroin regulates osteogenic cell behavior by modulating the stability of the adsorbed protein-material interface

Fig. 6

Osteogenic differentiation of BMSCs on different SF substrates. a, b Images of ALP staining (a) and quantitative detection of ALP activity (b) in BMSCs at day 7. c, d Images of ARS staining (c) and quantitative analysis of calcium concentration (d) in BMSCs at day 14. e, f Immunofluorescence images of collagen Ι (e) in BMSCs and quantitative analysis of fluorescence intensity of collagen Ι (f) at day 14. g–l mRNA expression levels of representative (g–h) Alp, (i–j) Osterix, and (k–l) collagen Ι in BMSCs at days 7 and 14. Error bars represent one standard deviation. (*P < 0.05 and **P < 0.01)

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