Fig. 5

Restoring ZBP1 expression rescues the osteogenic potential of ZBP1-depleted mBMSCs. a RT-qPCR and western blot analysis of the restoration of ZBP1 in ZBP-depleted mBMSCs. ALP activity (b) and ECM mineralization (c) were rescued by restoring ZBP1 expression in ZBP-depleted mBMSCs. d The expression of osteogenic markers (sp7, Ibsp, and Bglap) was rescued by restoring ZBP1 expression in ZBP1-depleted mBMSCs. *P < 0.05; **P < 0.01; ***P < 0.001