Fig. 3
Trabecular bone mass is only moderately affected in Wnt1G177C/G177C mice. a Representative undecalcified spine sections from 4-week-old male mice with the indicated genotypes (Wnt1G/G = Wnt1+/+, Wnt1G/C = Wnt1+/G177C and Wnt1C/C = Wnt1G177C/G177C) after von Kossa/van Gieson staining. b Quantification of the trabecular bone volume (BV/TV, bone volume per tissue volume) in female and male Wnt1+/+, Wnt1+/G177C, and Wnt1G177C/G177C mice at 4, 12, and 24 weeks of age. c Quantification of the osteoblast (N.Ob/B.Pm, number of osteoblasts per bone perimeter) and osteoclast numbers (N.Oc/B.Pm, number of osteoclasts per bone perimeter) in the same groups of mice. d Quantification of the bone formation rate (BFR/BS, bone formation rate per bone surface) in the trabecular compartment of the lumbar spine (top) and serum crosslaps (CTx) levels (bottom) of male and female Wnt1+/+, Wnt1+/G177C and Wnt1G177C/G177C mice at the age of 12 weeks. Data were analyzed by one-way ANOVA with Dunnett’s multiple comparison test (*P < 0.05, **P < 0.01, ***P < 0.001) and Student’s t-test (Wnt1G177C/G177C vs. Wnt1+/+, #P < 0.05, ##P < 0.01). Error bars indicate standard deviation
