Fig. 4
The WNT1G177C mutation primarily affects the quantity and quality of cortical bone. a Representative µCT images of the trabecular bone in the distal femoral metaphysis from 12-week-old female mice. Quantification of the trabecular bone volume in female and male Wnt1+/+ (G/G), Wnt1+/G177C (G/C), and Wnt1G177C/G177C (C/C) mice at 4, 12, and 24 weeks of age is given on the right. b Representative µCT images of the cortical bone in the central femoral diaphysis from 12-week-old female mice. Quantification of the cortical thickness in female and male Wnt1+/+ (G/G), Wnt1+/G177C (G/C), and Wnt1G177C/G177C (C/C) mice at 4, 12, and 24 weeks of age is given on the right. c Quantification of mechanical properties of the femora from 24-week-old female and male mice with the indicated genotypes as determined by 3-point bending (Fmax: Maximum force that the bone could withstand; WRp0.2: Work required to induce plastic deformation). d Quantification of the same parameters after normalization for morphological differences. e qBEI-based analysis of calcium distribution (top) and quantification of the calcium content (bottom) in the cortical compartment of tibiae from 24-week-old male wild-type and Wnt1G177C/G177C mice. Data were analyzed by one-way ANOVA with Dunnett’s multiple comparison test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.000 1) and Student’s t-test (Wnt1G177C/G177C vs. Wnt1+/+, #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.000 1). Error bars indicate standard deviation
