Fig. 5 | Bone Research

Fig. 5

From: Single cell analysis reveals inhibition of angiogenesis attenuates the progression of heterotopic ossification in Mkx−/− mice

Fig. 5

Differential gene expression analysis revealed elevated angiogenic gene expression in Mkx−/− tendon cells. a–c Heatmap of the normalized gene expression of DEGs between WT and Mkx−/− TPC (a), TB (b) and TC (c) clusters. d–f Representative GO terms corresponding to upregulated genes in the Mkx−/− TPC (d), TB (e) and TC (f) clusters. g Violin plots of proangiogenic genes, including Hif1a, Thbs1, Vegfa, and Flt1, in the TPC, TB and TC clusters. h Safranin O-Fast green staining of the patellar tendons from WT and Mkx−/− mice. The yellow arrows indicate the blood vessels. Scale bar, 50 μm. i Immunostaining of CD31 in WT and Mkx−/− patellar tendons. Scale bar, 50 μm. ns no significance; *P < 0.05; **P < 0.01; ***P < 0.001. TPCs tendon progenitor cells, TBs tenoblasts, TCs tenocytes

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