Fig. 6

AIB5P interacts with integrin α5 to promote osteogenesis. a Schematic diagram of the biotinylated peptide pulldown/MS assay. b Mass spectrometry analysis identified Itga5, which was pulled down from BMSC lysates by biotin-labeled AIB5P. c Representative western blots of Itga5 pulled down by biotin-labeled AIB5P. d qPCR analysis of siRNA-mediated Itga5 gene silencing efficiency. n = 3 e qPCR analysis of known osteogenic markers in AIB5P-treated, Itga5 knockdown BMSCs. n = 3 f ALP staining and g ARS staining of AIB5P-treated, Itga5 knockdown BMSCs. The experiment was repeated three times independently with similar results. h Representative western blots of FAK signaling component proteins in AIB5P-treated BMSCs. The experiment was repeated three times independently with similar results. i ALP staining and j ARS staining of AIB5P-treated BMSCs in the presence of the Itga5 inhibitor PF573228 (10 μmol·L⁻¹). The experiment was repeated three times independently with similar results. Bar, 100 μm. k Schematic diagram of AIB5P-related osteogenic pathways