Fig. 1
From: Targeting ferroptosis suppresses osteocyte glucolipotoxicity and alleviates diabetic osteoporosis
The diabetic microenvironment induced osteocyte ferroptosis in vivo. a A schematic illustration of the DOP model established by HFD feeding with injection of low-dose STZ. b Gross images of CTL and STZ&HFD mice. c Representative micro-CT (scale bar: 300 μm) and X-ray (scale bar: 2 μm) images showing the microarchitecture of the distal femur. Tibial paraffin sections stained with H&E (scale bar: 50 μm) and subjected to TUNEL (scale bar: 50 μm) showed empty lacunae and dead osteocytes. Quantitative analysis of the BV/TV ratio (d) trabecular separation (Tb.Sp, e), trabecular number (Tb. N, f), trabecular thickness (Tb. Th, g), number of empty lacunae with respect to bone area (N. Empt. Lc./B. Ar., h), fraction of empty lacunae (Frac. Empt. Lc., i) and number of TUNEL-positive cells (j). k Tibial paraffin sections stained for GPX4 and PTGS2 (scale bar: 50 μm). l The expression level of GPX4 in tibial bone tissue was determined by WB analysis. m Semiquantitative analysis of GPX4 expression based on WB analysis. Concentration of MDA in serum (n) and bone tissue (o). Serum concentrations of OCN (p), FFAs (q), and PA (r). *P < 0.05; **P < 0.01. Each group contained six mice
