Fig. 4
From: Targeting ferroptosis suppresses osteocyte glucolipotoxicity and alleviates diabetic osteoporosis
HO-1 was essential for HGHF-induced ferroptosis. a Representative images of HO-1-stained bone sections from CTL mice and STZ&HFD mice (scale bar: 50 μm). b Quantification of HO-1 expression in vivo. c Western blot showing HO-1 expression under various treatments. d Quantification of HO-1 expression based on WB analysis. e RT–qPCR showing HO-1 expression under various treatments. The β-actin gene was used as the internal reference gene. C11-BODIPY (scale bar: 100 μm) and FerroOrange (scale bar: 100 μm) staining of ZnPP-treated osteocytes (f) and HO-1-overexpressing osteocytes (g). h, i Quantification of fluorescence intensity. j Osteocyte viability was assessed by a CCK-8 assay. k The MDA concentration was assessed using an MDA assay kit. **P < 0.01. “NS” indicates nonsignificant. All data are from n = 3 independent experiments
