Fig. 7
From: Targeting ferroptosis suppresses osteocyte glucolipotoxicity and alleviates diabetic osteoporosis
Targeting ferroptosis rescued DOP and osteocyte death in diabetic mice. a, b Representative micro-CT (scale bar: 300 μm) and X-ray (scale bar: 2 μm) images showing the microarchitecture of the distal femur. c Quantitative analysis of the micro-CT parameters. d H&E staining of tibial paraffin sections showing empty lacunae (scale bar: 50 μm). e TUNEL assay of tibial paraffin sections showing dead osteocytes (scale bar: 50 μm). f, g Tibial paraffin sections stained for HO-1 and GPX4 (scale bar: 50 μm). h, i Quantitative analysis of the number of empty lacunae with respect to the bone area (N. Empt. Lc./B. Ar.) and the fraction of empty lacunae (Frac. Empt. Lc.) based on H&E staining. j Quantitative analysis of TUNEL-positive cells based on a TUNEL assay. k, l Quantitative analysis of HO-1 and GPX4 expression in vivo based on IHC staining. *P < 0.05; **P < 0.01. Each group contained six mice
