Fig. 2

Ly6ChiCD11bhi and Ly6ChiCD11blo cells give rise to OCs in vitro. a The isolation and sorting strategy for Ly6C^hiCD11b^hi and Ly6C^hiCD11b^lo cells is presented, and the purity was ≥98% in all samples. b Sorted Ly6C^hiCD11b^hi or Ly6C^hiCD11b^lo cells (10⁴) from the BM of control and inflamed mice were cultured with osteoclast differentiation medium for 4 and 8 days. Multinucleated TRAP-stained OCs appeared in Ly6C^hiCD11b^lo cultures after 4 days and in Ly6C^hiCD11b^hi cultures after 8 days (bar: 200 µm). c Area quantitation of OCs derived from Ly6C^hiCD11b^lo cells after 4 days and from Ly6C^hiCD11b^hi cells after 8 days in culture. b, c Control n = 5, inflamed n = 5, for each cell population; representative results for 5 independent experiments (Mann–Whitney test). Line: median, box: 25th–75th percentile, whiskers: range. *P < 0.05, **P < 0.01