Fig. 1
Isolation and identification of mesenchymal stem cells from mouse bone marrow. a Fibroblast-like or spindle-shaped morphology of mesenchymal stem cells appears at passages 4–6. b Passage 4–6 cells were harvested and stained with positive surface markers of mouse BM-MSCs: CD29, CD44, CD105, and Sca-1 and negative markers: CD31, CD34, CD45, CD86, and CD11b with their corresponding isotype control via FACS analysis. c Adipogenesis of MSCs was observed with oil red O staining, osteoblastogenesis was assayed with in situ alkaline phosphatase staining, and chondrocytic cells were identified with toluidine blue staining. d RT-PCR results showing the relative expression of bglap, runx2, alpl, acan and pparg with or without NH4Cl treatment
