Fig. 6
HO induction prohibits anti-angiogenesis phenotype. Single cell RNA sequencing analysis of pooled cells from the injury site, obtained at 0, 7 and 21 days post-injury. a UMAP visualization of seven cell clusters including mesenchymal progenitor cells (MPCs), pericytes, endothelial cells, pericytes, immune cells, Schwann cells, myoblast and lymphatic cells. b Violin plots demonstrating characteristic markers of each cell cluster. Pdgfra (Platelet-derived growth factor receptor alpha), Pdgfrb (Platelet-derived growth factor receptor beta), Acta2 (Actin alpha 2), Pecam1 (Platelet endothelial cell adhesion molecule 1), Ptprc (Protein tyrosine phosphatase receptor type c), Sox2 (SRY-box transcription factor 2), Sox10 (SRY-box transcription factor 10), Pax7 (Paired box 7), Lyve1 (Lymphatic vessel endothelial hyaluronan receptor 1). c Module score analysis of angiogenic inhibition across seven cell clusters. Gene lists for modular scores are shown in File S1. d UMAP visualization of expression of anti-angiogenic factors across 7 cell clusters. e Module score analysis of angiogenic inhibition across time. f Heatmap of 8 anti-angiogenic genes across time, including Ifng (Interferon gamma), Il18 (Interleukin 18), Serpinf1 (Serpin family F member 1), Timp2 (Timp metallopeptidase inhibitor 2), Il1b (Interleukin 1 beta), Pf4 (Platelet factor 4), Lect1 (Chondromodulin), and Tnni3 (Troponin I3). ** P < 0.01, non-parametric data was analyzed using the Kruskal–wallis with Dunn’s multiple comparison test (c, e)
