Fig. 2

Enhanced trabecular bone resorption in 3-month-old Adq-Gsα^R201C mice. a, b Representative histological pictures from Sirius red-stained sections and histomorphometric analysis of trabecular bone of lumbar vertebrae (a) and femora (b). BV/TV: bone volume per tissue volume. c TRAP histochemistry highlighting cells of the osteoclastic lineage (arrow) and histomorphometry of osteoclast parameters. N.Oc/BS: number of osteoclasts per bone surface. Oc.S/BS: osteoclast surface per bone surface. d Relative gene expression of Rankl, Opg, Rankl/Opg ratio and Rank in femora from female mice. e Representative images from H&E-stained sections showing osteoblasts (arrowhead) and histomorphometry of osteoblast parameters. N.Ob/BS: number of osteoblasts per bone surface. Ob.S/BS: osteoblast surface per bone surface. f Representative pictures of calcein labeling in trabecular bone of female mice and dynamic histomorphometry for bone formation parameters. MS/BS: mineralizing surface per bone surface. MAR: mineral apposition rate. BFR/BS: bone formation rate. g Relative gene expression of Alpl, Sp7 and Bglap in femora from female mice. F Females. M Males. Data are presented as mean ± SEM. Statistical analysis was performed using Student t-test; ^*P < 0.05, ^**P < 0.01, ^***P < 0.001