Fig. 8
Adq-intraosseous pericytes associate with lytic lesions in cortical and trabecular bone in Adq-GsαR201C mice. a Radiographic analysis of dissected tail vertebrae from 9-month-old Rosa26 and Adq-GsαR201C mice showing several osteolytic lesions (arrowhead). b Representative Sirius red stained sections of tail vertebrae from 9-month-old mice. c H&E-stained sections showing dissecting and tunneling resorption in tail vertebrae. Note the massive presence of osteoclasts (black arrow). d Sirius red and TRAP-stained sections showing intracortical bone resorption in tail vertebrae. Resorption areas progressed over time and were enriched in osteoclasts (black arrow) in close association with blood vessels (bv). e Immunolocalization of ALP, OSX and RANKL in a tail vertebra lytic lesion of Adq-GsαR201C mice. f Representative confocal images from Adq-mTmG;GsαR201C mice tail vertebrae showing areas of osteolysis (dashed line) filled with GFP-positive perivascular cells (arrow) and stromal cells (asterisk). g Schematic representation of lytic lesions associated with intracortical Gsα-mutated Adq-pericytes. cb: cortical bone, bt: bone trabecula, bm: bone marrow, gp: growth plate
