Table 1 Sequence of primers used for genotyping and qPCR
Genotyping | |
Mouse strain | Sequence 5’ − 3' |
Adipoq-Cre | F: GCATTGCTGTCACTTGGTCGT |
R: CGATGCAACGAGTGATGAGG | |
R26-lsl-GsαR201C | F: AAAGTCGCTCTGAGTTGTTAT |
R: GCGAAGAGTTTGTCCTCAACC | |
R: GGAGCGGGAGAAATGGATATG | |
R26-mTmG | F: CTCTGCTGCCTCCTGGCTTCT |
R: CGAGGCGGATCACAAGCAATA | |
R: TCAATGGGCGGGGGTCGTT | |
qPCR | |
Gene | Sequence 5’ − 3' |
Alpl | F: CCAGAAAGACACCTTGACTGTGG |
R: TCTTGTCCGTGTCGCTCACCAT | |
Bglap | F: GCAATAAGGTAGTGAACAGACTCC |
R: CCATAGATGCGTTTGTAGGCGG | |
Col1a1 | F: CAGGGTATTGCTGGACAACG |
R: TTGTTTGCCAGGTTCACCAGA | |
Gapdh | F: CATCACTGCCACCCAGAAGACTG |
R: ATGCCAGTGAGCTTCCCGTTCAG | |
HA (R26-Gnas) | F: GAAGAGGACGTGCCGGATTAC |
R: TGGTTTCAATGGCCTCCTTCA | |
Opg | F: CGGAAACAGAGAAGCCACGCAA |
R: CTGTCCACCAAAACACTCAGCC | |
Rank | F: GGACAACGGAATCAGATGTGGTC |
R: CCACAGAGATGAAGAGGAGCAG | |
Rankl | F: CGAGCGCAGATGGATCCTAA |
R: GCAGGAGTCAGGTAGTGTGT | |
Runx2 | F: CCTGAACTCTGCACCAAGTCCT |
R: TCATCTGGCTCAGATAGGAGGG | |
Sp7 | F: GGCTTTTCTGCGGCAAGAGGTT |
R: CGCTGATGTTTGCTCAAGTGGTC | |
