Fig. 6 | Bone Research

Fig. 6

From: Endothelial PDGF-BB/PDGFR-β signaling promotes osteoarthritis by enhancing angiogenesis-dependent abnormal subchondral bone formation

Fig. 6

Endothelial PDGFR-β promotes angiogenesis through the PDGFR-β/Talin1/FAK pathway. The efficiency of lentivirus transfection was verified using Western blot analysis (a) and qRT‒PCR (b). For pdgfrb silencing, 80958-1 exhibited optimum efficiency and was selected for the following experiments. c ELISA examination of VEGF expression in the CM of BMECs under different conditions. d–g Representative images and quantification analysis of tube formation in BMECs. Scale bar, 500 µm. h CCK-8 measurement of BMEC proliferation under different conditions. i, j Scratch wound assay showing BMEC motility under different conditions. Scale bar, 200 μm. Control = CM of BMECs from the normal control group; Over = CM of BMECs transfected with Lv-PDGFR-β; pdgfrb silencing = CM of BMECs transfected with Lv-si PDGFR-β; siTLN1 = CM of BMECs transfected with TLN1 siRNA; PF573228 = CM of BMECs treated with PF573228 (FAK inhibitor). *P < 0.05 and **P < 0.01 compared to the over group. #P < .05 and ##P < .01 compared to the control group

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