Fig. 7
Activation of tumor-associated macrophages (TAMs) and osteoclasts (OCs) in OS. a UMAP plot of myeloid cells, including OCs, using the Monocle3 method. b UMAP plot of myeloid cells color-coded for each OS and CB sample. c Pseudotime trajectory of myeloid cells. d Differences in cell proportion between OS and CB samples in myeloid cell clusters. e Violin plots showing differences in expression of monocyte, M1Φ, M2Φ, TAM, and OC markers in each myeloid cluster. f Dot-plot heatmap of the most significant genes in the five myeloid clusters. g Heatmap showing differences in pathway activation between the five myeloid clusters calculated by the GSVA method. h Violin plots showing differences in activation of important pathways between the five myeloid clusters, including OC development, bone resorption, macrophage activation involved in immune response, regulation of antigen processing and presentation, tricarboxylic acid metabolic process, positive regulation of sprouting angiogenesis, positive regulation of fibroblast migration, and regulation of bone development pathways (part of the pathway is abbreviated in the figure)
