Fig. 6
Images of healthy and osteoporotic bone in a rodent model developed in normogravity. A greater level of adiposity is observed within the osteoporotic architecture. a MicroCT images showing that the trabecular architecture is slowly resorbed, leading to alterations in pore number, size and shape. b Histological images demonstrate narrowing of the trabeculae (T) and the generation of an increased level of lipids in osteoporotic bone compared with healthy tissue. Sudan Black B stained phospholipids (gray) and intracellular lipids (black). c Perilipins are found exclusively on adipocytes, and using immunohistochemistry (IHC), a greater amount of positive staining is observed within the marrow of osteoporotic bone. Positive lipid staining is indicated by red arrows
