Fig. 6
Deficiency of the Piezo1 channel attenuates IVDD development through GPX4. a Flowchart of animal experiments on Piezo1-cKO、Gpx4-cKO、Piezo1/Gpx4-cKO mice. b The X-Ray, MRI and Micro-CT images for coccygeal disks of mice after needle puncture. c Quantitative analysis of X-ray、MRI and Micro-CT (n = 3). d The Safranine O-Fast Green staining of WT, Piezo1-cKO, Gpx4-cKO and Piezo1/Gpx4-cKO mice. Scale bar, 250 μm. e The immunohistochemical assay of ACSL4, COL2 and Aggrecan of mice. Scale bar, 250 μm. f–h Immunofluorescence staining analysis of ACSL4、DMT1、FSP1 in different mice. Scale bar, 200 μm. i Quantitative analysis of the immunofluorescence staining, Safranine O-Fast Green staining and immunohistochemical assay (n = 3). All data are expressed as the mean ± SEM, n = 3 replicates from one representative of 3 independent experiments. ns (no significance), *P < 0.05, **P < 0.01, ***P < 0.001
