Fig. 2

HIF-1α regulates collagen ECM organization to stimulate trauma-induced HO. a An scRNA-Seq analysis and integrated UMAP cluster and cluster definition at day 0, 7, and 42 post-BT of Hoxa11-CreER^T2; ROSA-LSL-TdTomato mice. b Gene ontology of upregulated DEGs was analyzed using web-based Metascape. c Violin plot of relative expression of Hif1a across the time points (D0, D7, and D42) post-BT injury of Hoxa11-CreER^T2; ROSA-LSL-TdTomato mice. D0 vs. D7, adjusted P value = 0; D0 vs. D42, adjusted P value = 4.62E-33. d Violin plots of relative expression of Lox, Loxl1, and Loxl3 across the time points (D0, D7, and D42) post-BT injury of Hoxa11-CreER^T2; ROSA-LSL-TdTomato mice. Lox (D0 vs. D7, adjusted P value = 0; D0 vs. D42, adjusted P value = 1.06E-42); Loxl1 (D0 vs. D7, adjusted P value = 1.79E-56); Loxl3 (D0 vs. D7, adjusted P value = 1.38E-123). e SpatialFeaturePlot (left) and dot plot (right) showing expression of genes involved in HIF-1α signaling. f An scRNA-Seq analysis and integrated UMAP cluster and cluster definition at day 7 post-BT of Hoxa11-CreER^T2 (+); Hif1a^fl/fl and littermate control Hoxa11-CreER^T2 (-); Hif1a^fl/fl mice. g Gene ontology of DEGs downregulated in Hoxa11-CreER^T2 (+); Hif1a^fl/fl mice 7 days post-BT injury. h Modular scoring for the HIF-1 pathway in MPC cluster from Hoxa11-CreER^T2 (−); Hif1a^fl/fl mice and Hoxa11-CreER^T2 (+); Hif1a^fl/fl mice 7 days post-BT. t test **P = 0.004. i Violin plots of relative expression of collagen cross-linking enzymes in Hoxa11-CreER^T2 (−); Hif1a^fl/fl and Hoxa11-CreER^T2 (+); Hif1a^fl/fl mice 7 days post-BT. Lox (adjusted P value = 0.002); Loxl1 (adjusted P value = 2.67E-11); Loxl3 (adjusted P value = 1.31E-25)